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PMI is a safe and environmentally-friendly
positive selection system for plant transformation, providing a novel alternative
to antibiotic and herbicide resistance markers for a number of commercially
important crops and model plant species. Through the links below you can obtain
information on the relevant patents and technical aspects of the system and
its applications.
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More information about PMI
Relevant publications:
- Datta, K., et al. 2003. Plant Biotechnology Journal 1:81-90.
Bioengineered golden indica rice cultivars with beta-carotene
metabolism in the endosperm with hygromycin and mannose selection systems.
- Todd, R. 2001. Plant Molecular Biology Reporter 19:307-319.
Phosphomannose isomerase: A versatile selectable marker for Arabidopsis
thaliana germline transformation.
- Negrotto, D., et al. 2000. Plant Cell Reports 19:798-803.
The use of phosphomannose isomerase as a selectable marker to recover
transgenic maize plants (Zea mays L. via Agrobacterium transformation.
- Joersbo, M., et al. 1996. Plant Cell Reports 16:219-221. A
novel principle for selection of transgenic plant cells: positive selection.
Relevant issued patents:
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The Hygromycin selectable marker system involves
use of the hygromycin phosphotransferase gene (HYG) as the marker gene, which
provides for resistance to hygromycin as the selective agent in plant transformation.
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Relevant publications:
- Liau, C.-H., et al. 2003. Plant Cell Reports 21:993-998. Agrobacterium
tumefaciens-mediated transformation of an Oncidium orchid.
- Olhoft, P., et al. 2003. Planta-Berlin 216:723-735.
Efficient soybean transformation using hygromycin B selection in
the cotyledonary node method.
- Kuvshinov, V., et al. 1999. Plant Cell Reports 18:773-777. Agrobacterium
tumefaciens-mediated transformation of greenhouse grown Brassica
rapa ssp. Oleifera.
Relevant issued patents:
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For inquiries about licenses for commercial organizations, contact
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