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The Barton and Chilton method is a method for
producing genetically engineered plant cells using a disarmed Ti plasmid.
Regenerated fertile plants can be recovered from plants cells transformed with
T-DNA delivered by Agrobacterium tumefaciens.
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Relevant publications:
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Chilton, M.-D. 1983. Scientific American 248:50-59. A vector for
introducing new genes into plants.
Relevant issued patent:
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The Binary Vector System (BVS) is one of the
simplest and most reliable vector systems for Agrobacterium transformation
of dicotyledonous plants. Use of BVS offers the advantage of direct cloning in E.
coli, with vectors carrying multiple cloning sites, markers for direct
screening of insertions, and expression cassettes for the transgene.
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Relevant publications:
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Zhu, J., et al. 2000. Journal of Bacteriology
182:3885-3895. The bases of crown gall tumorigenesis.
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Hooykaas, P.J.J., et al. 1994. Annual Review of Phytopathology 32:157-180.
The virulence system of Agrobacterium tunefaciens.
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Hooykaas, P.J.J., et al. 1985. Trends Biochem. Sci. 10:307-309.
The Ti-plasmid of Agrobacterium tunefaciens: a natural genetic engineer.
Relevant issued patents:
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